Osteoporosis: Methods and Protocols by Thomas A. Owen, Lydia C. Pan (auth.), Jennifer J. Westendorf

By Thomas A. Owen, Lydia C. Pan (auth.), Jennifer J. Westendorf (eds.)

In fresh years, the learn of bone cells and tissues on the mobile and molecular degrees in a number of types has revolutionized the sector. In Osteoporosis: equipment and Protocols, major scientists from world wide proportion their step by step laboratory protocols for learning bone biology. the subjects lined during this quantity comprise in vitro types, in vivo versions applied for drug checking out, tissue engineering and osteoporosis reviews in both gender, state of the art molecular suggestions to evaluate unmarried genes or for international genomic research, strong imaging strategies, and lots of extra. As a quantity within the hugely profitable Methods in Molecular Biology™ sequence, every one bankruptcy presents a short advent, an inventory of useful fabrics, and a Notes part detailing tips about troubleshooting and averting identified pitfalls.

Comprehensive and state-of-the-art, Osteoporosis: equipment and Protocols is the proper advisor for either new and skilled experimentalists trying to learn the devastating silent ailment referred to as osteoporosis.

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2. Rinse slices repeatedly with sterile water and air-dry in a laminar flow hood. 3. Place slices into 48-well plates and add non-adherent precursor cells as in the above RANKL/M-CSF culture protocol. 4. On day 3, move the slices to a six-well plate for final differentiation (see Note 14). 5. Depending on the experimental design, treatment will commence when precursors are plated, on day 3, or when the osteoclasts are mature on day 4. For in vivo generated osteoclasts directly seeded onto bone, after an overnight incubation, slices with adherent osteoclasts are transferred to six-well plates and treatment commenced (see Note 14).

Pick the first pup with the large forceps. 6.

References 1. , et al. (1997) Regulation of osteoclast function. J Bone Miner Res 12, 869–879. 2. , et al. (1990) Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells. Proc Natl Acad Sci U S A 87, 7260–7264. 3. , et al. (1999) Commitment and differentiation of osteoclast precursor cells by the sequential expression of c-Fms and receptor activator of nuclear factor kappaB (RANK) receptors.

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