Microscopy from the very beginning by Friedrich K. Mollring

By Friedrich K. Mollring

Microscopy from the Very starting

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This will effectively reduce ihe danger of bubbles forning when the condenser is immersed. Wheiher both oil films are aciually free lrom bubbles can easily be determined by looking into the objective from the top afier having removed ihe eyepiece. raphylococc {cram s sia n) 42 8. cal power of ihe cover glass was t a k e n l n t o a c c o u n tl n t h e c o m p u t a t o n a n d d e s i g n o f i h e o b j e c t v e . Nature), which is l o c a t e do u t s i d et h e o b j e c t l v em o u n t b u t s t f o r n i s a p a r t o f t h e o p t i c a ls y s t e r n - T h e o p u m l m t h l c k n e s so f a c o v e f T h e h i g h e r l h ea p e r t u r e t h e .

O- I - Ta. o€ "q- reo w h e n d r y o b l e c t l v e sa f e u s e d . m m e r s i o no i l , w h i c h i n t h e case of an o imnrersor obtectve f s ihe space between t h e c o v e r q l a s s a n d t h e f r o i t e n s h a s a r e f r a c t i v en d e x o f 1 . 5 1 5 ,a . d i i s o b v o u s t h a t s ! mpned ! :lj ta 39 W h a t j s t h e r e s o l v i n gl i r n i t ? W i i h i h e a i d o f i h e n u m e r i c aal p e r i u r ew e c a n a l s o c o m p u t e ihe limjt of resolution or maximlm resolving power.

Obt + n. a. oond E x a m p l e : U s i n g a g r e e n f i l t e r , t h e w a v ee n g t h i s 0 - 5 5 p ; a s s u m i n gt h e o b j e c t i v eu s e di o h a v ea n a p e r t u r eo f 1 . 2 5 ,a n d t h e c o n d e n s e ro n e o f 0 - S rt h e n d = 0 . 2 5r r , i . e . , t h e f o u r t h part of a thousandth of a millimeter. llfidelity. 4A 7. Oil immersion Whatis oil immersion? F o r t h e s a m e i m a g e s c a l e , a n o i l i m m e r s i o no b j e c t i v e i s a L w a y s s u p e r i o r t oa c o m p a r a be d r y o b j e c t v e a s r e g a r d s c o r r e c t i o n .

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