By Ayse Elif Erson-Bensan (auth.), Malik Yousef, Jens Allmer (eds.)
In miRNomics: MicroRNA Biology and Computational Analysis, professional researchers within the box current an outline of the present cutting-edge and goal to place the respective parts of analysis right into a greater standpoint. those contain tools and methods starting from miRNA biogenesis, their organic functionality, computational analyses to their scientific implications and functions. Written within the hugely winning Methods in Molecular Biology series layout, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and key tips about troubleshooting and keeping off identified pitfalls.
Authoritative and useful, miRNomics: MicroRNA Biology and Computational research seeks to assist scientists within the extra research into miRNA examine and statistics.
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Additional info for miRNomics: MicroRNA Biology and Computational Analysis
Using an EC specific Dicer knockout mice model . According to this model, there were no distinct differences in the vascular phenotypes between both mice models. Moreover, studying the function of Dicer by specific knockdown experiments resulted in reduced proliferation and endothelial sprouts formation. However, knocking down Dicer interestingly resulted in the overexpression of a pro-angiogenic factor via Thrombospondin-1 upregulation . In addition to Dicer, Drosha was also knocked down which resulted in decreased angiogenesis in human ECs in vitro.
Krichevsky AM, King KS, Donahue CP et al (2003) A microRNA array reveals extensive regulation of microRNAs during brain development. RNA 9:1274–1281 22. Smirnova L, Grafe A, Seiler A et al (2005) Regulation of miRNA expression during neural cell specification. Eur J Neurosci 21:1469–1477 23. Schratt GM, Tuebing F, Nigh EA et al (2006) A brain-specific microRNA regulates dendritic spine development. Nature 439:283–289 24. Sokol NS (2012) The role of microRNAs in muscle development. Curr Top Dev Biol 99:59–78 25.
The transcription factor c-MYC is regulating genes that have roles in development, cell proliferation, differentiation, and apoptosis, and control the cell cycle, regulating G1–S transition. In some cancers, c-MYC was found to be deregulating miRNA expression . , c-MYC induces expression of the mir-17-92 cluster which was considered as an oncogene since it accelerates tumor development in a B-cell lymphoma animal model . Moreover, mir-106a-92-2 is the paralogous cluster of mir-17-92 and its transcription is induced by MYCN in neuroblastoma cells .