Methods in Soil Biology by F. Schinner (auth.), Prof. Dr. Franz Schinner, Dr. Richard

By F. Schinner (auth.), Prof. Dr. Franz Schinner, Dr. Richard Öhlinger, Doz. Dr. Ellen Kandeler, Dr. Rosa Margesin (eds.)

In terrestrial ecosystems, soil microorganisms and soil animals are crucial for muddle degradation, soil formation and the supply of foodstuff and hint parts. The size of organic soil parameters permits a swift review of the results of chemical and actual affects as a result of toxins or soil management.
This publication introduces a few good proved tools for the research of carbon, nitrogen, phosphorus and sulfur cycles. It focuses extra at the selection of the quantity and biomass of microorganisms, algae and animals within the soil. specific emphasis is put on the understandable and whole description of the experimental procedures.

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From the number of tubes in three subsequent dilutions showing ethylene formation, the most probable number of N2-fixing bacteria in the soil sample is calculated. 2 ("Plate Count Technique") • Hot-air sterilized test tubes (15 x 160mm) with aluminium caps • Rubber serum stoppers (Schlitt, Gottingen or Arthur H. 2%) Dissolve 1 g of sodium polyphosphate (NaP0 3)n in distilled water, and dilute the volume to 1000ml with distilled water in a volumetric flask. 2mM) of KH zP0 4 in distilled water, add 1 ml of iron chelate stock solution and 1 ml of micronutrient stock solution (for stock solutions refer to Sect.

Store the solution in an amber bottle. 1 ml of nutrient solution from incubated culture tubes by means of a piston-stroke pipette to a spot plate, add 1 drop of solution A and then 1 drop of solution B. Red colouration shows the presence of nitrite. Rinse pipette tip before testing another tube. Alternatively, test strips for detection of nitrite (Merck 10007) may be used. Procedure Preparation of the Soil Suspension During all operations take precaution to exclude entry of contaminants into sterilized vessels and avoid contact of the interior with non-sterile objects.

50 Indirect Estimation of Microbial Biomass Allow to cool, and add 5ml of distilled water. Empty the clear supernatant into a separatory funnel, and wash the residue twice with 20 ml methanol. Empty the washing solution into the separatory funnel. After shaking out for 1 min with 30ml n-hexane, dry the hexane phase at 40°C in the rotary evaporator. Dissolve the residue in 2 ml methanol. After filtration of the solution through a membrane filter, determine the ergosterol content on HPLC by using a reversed-phase C-18 column, 95% methanol as mobile phase, and a flow rate of 1 ml min-I.

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