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And Wilson, G. 1986. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc. Natl. Acad. Sci. A. 83:9070-9074. , and Hanahan, D. 1988. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucl. Acids Res. 16:1563-1575. W. 1985. Construction and screening of cDNA libraries in λgt10 and λgt11. M. ) pp 49-78. IRL Press, Oxford. Russel, M. and Model, P. 1984. Replacement of the flp gene of Escherichia coli by an inactive gene cloned on a plasmid.
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7C). 5. The choice of reporter gene will depend on the cell type or organism, whether the assay will be done in vivo or in vitro, and whether quantitative or qualitative data is desired. The replicator, selectable marker, and other elements of the reporter vector will also have to be compatible with the system. PLASMID VECTORS FOR YEAST Yeast plasmid vectors contain the same basic features as E. 4). There are two primary types of replicators used in yeast plasmid vectors, autonomously replicating sequences (ARS) derived from the yeast chromosomes and the natural yeast 2µm plasmid replicator.