International Review of Cytology, Vol. 3 by G.H. Bourne, J.F. Danielli (Eds.)

By G.H. Bourne, J.F. Danielli (Eds.)

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CARBOHYDRATES AND OXYGEN The majority of the balanced salt solutions used in preparing and diluting tissue culture media have been fortified with glucose. The principal exceptions are Ringer’s (1886) solution, which was not designed for tissue culture but has often been used as a diluent ; and Drew’s (1922, 1923) and Pannett and Compton’s (1924) solutions, which were developed for use in tissue culture media. By far the most commonly used formula has been that of Tyrode (1910). /100 ml. glucose, which is close to the average normal mammalian blood sugar level.

H e found that, of the three hexoses tested, mannose was superior to glucose or fructose for maintaining the life of the cultures, and that proliferation of both fibroblasts and epithelium took place. Glucose, and still more so fructose, favored epithelium rather than fibroblasts. Glycogen, lactate, dihydroxyacetone, pyruvic aldehyde, and glucosamine failed to support proliferation, though heart beat persisted for many days in the presence of lactate. Phosphorylated hexoses were not superior to mannose, but were utilized.

0% were in general inhibitory to growth or productive of cell granularity. Latta and Bucholz (1939) reported that 1 to 2% glucose inhibited, and 5% stopped, fibroblast growth in vitro without affecting embryonic muscle migration. 5%. It is evident that cells in tissue culture can tolerate, at least for a short time, concentrations of glucose much higher than physiological. However, high concentrations of the order found optimum by Ebeling (1936) have not often been used. /100 ml. glucose beneficial in a medium (Kendall’s medium) composed of an extract of hog intestine in a buffered saline solution.

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