By Jeffrey Prichard (auth.), Fan Lin, Jeffrey Prichard (eds.)
In a conceptually present, quick-reference, query & solution layout, the guide of useful Immunohistochemistry: commonly asked Questions offers standardization of the immunostaining approach for every antibody and for every staining panel. With hyperlinks to the authors Immunohistochemical Laboratory site, this quantity creates a present and up to date info approach on immunohistochemistry. This contains entry to tissue microarrays (TMA) of over 5,000 tumors to validate universal diagnostic panels and supply the easiest reproducible facts for diagnostic purposes.
Chapters are provided in a distinct query and resolution structure. One table/IHC panel is equipped to deal with every one query. A concise explanatory observe follows each one table/panel to prevent diagnostic pitfalls. web site hyperlinks are supplied all through to replace the big details during this box, offering the most up-tp-date wisdom and the opportunity of stay specialist session. All chapters are written through nationally/internationally famous specialists within the comparable zone making sure authority and excellence.
Comprehensive but functional and concise, the instruction manual of sensible Immunohistochemistry: commonly asked Questions, might be of significant price for surgical pathologists, pathology citizens and fellows, cytopathologists, and cytotechnologists.
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Additional info for Handbook of Practical Immunohistochemistry: Frequently Asked Questions
The rate of reagent delivery rarely is the bottleneck in the process, because the robotic components that distribute reagents to the slides only work intermittently in between periods of reagent incubation. In this sense, having a large slide capacity can improve throughput by having more slides staining at a time. But of course it is not as simple as just slide capacity and protocol length. J. Prichard et al. Consider that there can be great variation in the time required for different slides in a batch to complete their staining cycle.
National Committee on Clinical Laboratory Standards MM7-A. Fluorescence in situ hybridization (FISH) methods for medical genetics; approved guideline. Wayne, PA: National Committee on Clinical and Laboratory Standards; 2004. Williams JH, Mepham BL, Wright DH. Tissue preparation for immunocytochemistry. J Clin Pathol. 1997;50(5):422–8. Yaziji H, Taylor CR. Begin at the beginning, with the tissue! The key message underlying the ASCO/CAP task-force guideline recommendations for HER2 testing. Appl Immunohistochem Mol Morphol.
Pathologist and histotechnologist: a marriage in need of counseling. Pathol Annu. 1975;10:205–11. Buesa RJ. Histology aging workforce and what to do about it. Ann Diagn Pathol. 2009;13(3):176–84. Buesa RJ. Staffing benchmarks for histology laboratories. Ann Diagn Pathol. 2010;14(3):182–93. Buesa RJ. Productivity standards for histology laboratories. Ann Diagn Pathol. 2010;14(2):107–24. Hsi ED. A practical approach for evaluating new antibodies in the clinical immunohistochemistry laboratory.