By Craig W. Stevens (auth.), Craig W. Stevens (eds.)
G Protein‐Coupled Receptor Genetics: study and strategies within the Post‐Genomic Era good points sensible recommendations encouraged through the short relocating GPCR box. From strong bioinformatic instruments tracing the evolution of GPCRs, to tools for the mobile transfection of engineered viruses containing GPCRs, to optogenetic concepts that produce light-activated GPCRs in reside mice, what was technological know-how fiction is now technological know-how truth. This unique quantity comprises sections masking genetic mechanisms, a genetic toolbox for GPCR discovery, in addition to genetic points of G protein-coupled receptors in future health and medication. Written for the Methods in Pharmacology and Toxicology sequence, this e-book includes the type of key implementation recommendation that encourages winning ends up in the lab.
Authoritative and straightforward to exploit, G Protein‐Coupled Receptor Genetics: study and techniques within the Post‐Genomic Era serves as an excellent consultant for researchers aiming to proceed our development during this dynamic and fascinating quarter of study.
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Extra info for G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era
1 High-Throughput Sequencing Projects The availability of high-throughput (HTP) sequencing technologies and continuously decreasing costs of sequencing produced a wealth of genome sequences. Whole-genome shotgun sequencing with Illumina or 454 technologies is currently used, but, after assembly of sequence fragments, proper gene annotation is required . Currently, three major lines of HTP sequence data sets are produced: (1) genome data from different species, (2) genome data from different individuals of one species (population genetic data), and (3) transcriptome data from different species, individuals, and cell/tissue types.
Biochim Biophys Acta 1772:1004–1021 Garcia MI, Fernandez-Vallone V, Vassart G (2014) Discovery and characterization of leucine-rich repeat-containing G proteincoupled receptors. In: Stevens CW (ed) Genetics of G protein-coupled receptors – research and methods in the post-genomic era. Methods in Pharmacology and Toxicology, Humana Press/Springer Science+Business Media, LLC, New York, pp 365–388 Styrkarsdottir U, Thorleifsson G, Sulem P et al (2013) Nonsense mutation in the LGR4 gene is associated with several human diseases and other traits.
However, this process requires an 38 Claudia Stäubert et al. intermediate variant which may be functionally inactive and is therefore eliminated from the gene pool (a “dead-end” intermediate). Further, in some GPCR variants, even synonymous substitution may have a disastrous effect at mRNA or translational levels (mutational induction of splice sites, change of RNA folding and stability, species-specific codon usage). Nevertheless, recent advances in gene synthesis technology, in high-throughput sequencing, as well as in high-throughput assays have made it possible to generate and functionally test comprehensive mutant libraries.