By V. Gianinazzi-Pearson, D. van Tuinen, E. Dumas-Gaudot, H. Dulieu (auth.), Professor Dr. Bertold Hock (eds.)
Fungal institutions Hock (Ed.) The 9th quantity contains: Mycorrhizae, Lichenes, Fungal Bacterial Interactions, and additional Fungal Interactions.
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1991), Medicago truncatula (Calantzis et a1. 1998), Lotus japonicum (Wegel et al. 1998) or Lycopersicum esculentum (Barker et a1. 1998). This kind of approach will however, be, very difficult for the fungal part, since all their developmental stages are coenocytic, and different nuclei in one cytoplasm might easily complement each other. An alternative approach is to target the function of a gene in a certain metabolic pathway. Following this type of strategy, genes could be cloned by complementing the corresponding mutants in a heterologous organism.
1987) obtained only two mAb-secreting cell lines from two spleens using soluble AM fungal antigen. This difficulty in obtaining mAb has also often been described for nonmycorrhizal fungal antigens. For example, out of 16 fusions of murine spleens immunized with the pathogen Pseudocer- 31 cosporella herpotrichoides, only 3 cell lines could be derived that expressed mAb which specifically recognized the antigen (Dewey et al. 1991). Schulze and Bahnweg (1998) describe poor immunogenic responses against fungal antigens in the production of mAbs for the identification and detection of Armillaria spp.
Using this method, root exudates and fractions of exudates have been analysed for their influence on hyphal morphology (Buee et al. 1998; Nagahashi et al. 1998). Adding this compound to in vitro germination assays of Scutello~pora castanea P. Franken and N. Requena 24 resulted in en-hanced hyphal branching (Table 1). In all the different analyses, the final identification of the responsive substances has still to be ascertained. Table 1. Influence of an unknown compound from root exudates on pre symbiotic stages of Scutellospora castanea Order of branches No.