Flow Cytometry Protocols by Teresa S. Hawley, Robert G. Hawley

By Teresa S. Hawley, Robert G. Hawley

Absolutely revised and up-to-date, move Cytometry Protocols highlights the increasing contribution of circulation cytometry to easy organic learn and diagnostic medication. The textual content offers state-of-the-art issues, equivalent to polychromatic, quantitative, and excessive throughput circulate cytometry, and extra.

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Methods 35, 167–176. 2. , Stephens, P. , Hewitt, C. , Powell, J. , and Badley, R. A. (2000) Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods 42, 97–114. Multiparameter Flow Cytometry of Bacteria 43 3. Shapiro, H. M. (2000) Microbial analysis at the single-cell level: tasks and techniques. J. Microbiol. Methods 42, 3–16. 4. Shapiro, H. M. (2003). Practical Flow Cytometry, 4th edit. Wiley-Liss, Hoboken, NJ. 5. Haugland, R. P.

1. When one univariate histogram is plotted on the x-axis and a second univariate histogram is plotted on the y-axis, as shown in Fig. 6, a bivariate histogram results. 2. If the two univariate histograms are each projected into the central area of this two parameter display, the values in common produce clusters of the cells shown by the dots. Each dot represents a cell and there are many overlapping dots for cells sharing common properties that produce a cluster. These clusters can be of any size and shape, depending on the relationship between the two properties that were measured by the antibodies.

V. (1991) Introduction to Flow Cytometry. Cambridge University Press, Cambridge. 18. Watson, J. V. (1992) Flow Cytometry Data Analysis: Basic Concepts and Statistics. Cambridge University Press, Cambridge. 19. Robinson, J. , Dean, P. , eds. (1997) Current Protocols in Cytometry. John Wiley & Sons, New York. 20. Diamond, R. A. , eds. (2000) in Living Color: Protocols in Flow Cytometry and Cell Sorting. Springer-Verlag, Berlin. 21. Durack, G. and Robinson, J. , eds. (2000) Emerging Tools for Single-Cell Analysis, Wiley-Liss, New York.

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