By Patrick M. McCue, Edward L. Squires
This booklet in brief stories the background of equine embryo move, overlaying in clinically functional phrases the recommendations, gear, and administration protocols presently in use. Embryo move has turn into a major company, specifically for breeding racing inventory (horses and camels), and is accordingly a vital point of equine perform. Ed Squires and Pat McCue were concerned with the improvement of embryo assortment and move methods because the early 60s and feature either contributed vital strategies and concepts to the method via their examine and scientific event. This ebook captures the scientific event, to date, and applies it on to equine perform. The booklet is of significant worth to normal equine practitioners for reference, equine copy experts, animal technology on the graduate point (equine track), and breeders.
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Extra resources for Equine embryo transfer
Mares that accumulate fluid in their uterus after insemination should be treated (see below) to optimize embryo recovery. Embryo collection should be scheduled based on the known ovulation date(s) and other factors, such as age of the donor mare, type of semen used, and information from previous embryo collection attempts. Embryo collection is usually performed on day 7 or day 8 after ovulation, although some clinicians prefer to flush a mare on day 9 and recover larger embryos. e. < 300 µm) for cryopreservation.
A square embryo search dish with grid on bottom. 48 Figure 6-15. Creating a grid on the bottom of a round plastic petri dish using a scalpel blade. Figure 6-16. Rinsing the filter with flush medium. Figure 6-17. Swirling the search dish moved cellular debris and hopefully the embryo into the center. CHAPTER 6 EMBRYO COLLECTION 49 If an embryo is not immediately located, the entire dish should be systematically searched using the grid lines for orientation. Debris may be moved out of the way using a sterile needle or other small sterile device (Figure 6-18).
Embryo collection can be successful early in the post-partum period, but the flush procedure is often associated with a large amount of uterine debris. It may be better to allow the mare to go through her foal heat, administer prostaglandins approximately 5 days after ovulation and breed the mare for embryo collection on the subsequent estrus or wait until the second post-partum estrus (30-day heat). 32 Recommended Reading LeBlanc MM. When to refer an infertile mare to a theriogenologist. Theriogenology 2008; 70: 421-429.