Electroporation and Sonoporation in Developmental Biology by Harukazu Nakamura Phd (auth.), Harukazu Nakamura Phd (eds.)

By Harukazu Nakamura Phd (auth.), Harukazu Nakamura Phd (eds.)

In ovo electroporation is an epoch-making success within the learn of developmental biology. With this system, experiments should be conducted in achieve and lack of functionality in wanted tissue at any wanted degree in chick embryos. creation of a tetracycline-regulated gene expression process and a transposon method has extra prolonged the potential for the strategy, making it attainable to acquire long term expression and to show off and on a gene of curiosity. it truly is now utilized to mice, aquatic animals, or even to vegetation for the examine of developmental biology and for different reasons. during this booklet, the applying of electroporation in lots of embryonic tissues and organs is brought, with a few chapters that take care of gene move in adults. Sonoporation, one other useful gizmo, utilizing ultrasonic waves rather than electrical currents, for gene move to mesenchymal tissues is usually brought.

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1990). A mouse embryonic stem cell line showing pluripotency of differentiation in early embryos and ubiquitous beta-galactosidase expression. Cell Differ Dev 29, 181–186. , Nakamura, H. (2003). The role of Grg4 in tectal laminar formation. Development 130, 451–462. , Nakajima, K. (2001). Efficient in utero gene transfer system to the developing mouse brain using electroporation: Visualization of neuronal migration in the developing cortex. Neuroscience 103, 865–672. , Tachibana, S. (2001). The use of ultrasound for drug delivery.

2004). Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison. Mech Dev 121, 1145–1158. , Hasan, M. , Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci U S A 97, 7963–7968. , Takahashi, Y. (2007). Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos.

2007). In contrast to pCAGGS-DsRed2, EGFP signals generated by pT2K-CAGGS-EGFP are retained for a prolonged period of time because of the Tol2-mediated genomic transposition (Fig. , 2007). The stable expression of EGFP indeed requires the activity of Tol2-transposase because electroporation with pT2KCAGGS-EGFP without pCAGGS-T2TP did not yield the retained expression. Persistent expression of EGFP in somite-derived tissues was observed until E8, the latest stage examined in our hands. , unpublished data).

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