Current Protocols in Molecular Biology - Chapter 11

Read or Download Current Protocols in Molecular Biology - Chapter 11 Immunology PDF

Best biology books

Osteoporosis: Methods and Protocols

In recent times, the learn of bone cells and tissues on the mobile and molecular degrees in various versions has revolutionized the sector. In Osteoporosis: tools and Protocols, top scientists from around the globe percentage their step by step laboratory protocols for learning bone biology. the subjects coated during this quantity contain in vitro types, in vivo types applied for drug trying out, tissue engineering and osteoporosis reviews in both gender, cutting-edge molecular concepts to evaluate unmarried genes or for worldwide genomic research, strong imaging innovations, and plenty of extra.

Molecular Mechanisms in Bioenergetics

This publication summarises present wisdom of the constitution, function,biosynthesis and law of energy-transducing enzymes inmitochondria, chloroplasts and micro organism. all the twenty chapters is written via best specialists of their box, and Prof. Ernster has ensured that the publication as a complete provides a well-integrated photo of the current country of information of the sphere at its various degrees and complexities.

Extra info for Current Protocols in Molecular Biology - Chapter 11 Immunology

Example text

And Wilson, G. 1986. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc. Natl. Acad. Sci. A. 83:9070-9074. , and Hanahan, D. 1988. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucl. Acids Res. 16:1563-1575. W. 1985. Construction and screening of cDNA libraries in λgt10 and λgt11. M. ) pp 49-78. IRL Press, Oxford. Russel, M. and Model, P. 1984. Replacement of the flp gene of Escherichia coli by an inactive gene cloned on a plasmid.

Nucl. Acids Res. 16:4465-4482. G. 1987. DNA methylation in Escherichia coli. Ann. Rev. Genet. 21:113-132. Miller, J. 1972. Experiments in Molecular Genetics. Y. H. 1978. The lacI gene: Its role in lac operon control and its uses as a genetic system. In The Operon (J. ) pp. 31-88. Y. W. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucl. Acids Res. 17:3469-3478. H. 1986. Factors which equalize the representation of genome segments in recombinant libraries.

7C). 5. The choice of reporter gene will depend on the cell type or organism, whether the assay will be done in vivo or in vitro, and whether quantitative or qualitative data is desired. The replicator, selectable marker, and other elements of the reporter vector will also have to be compatible with the system. PLASMID VECTORS FOR YEAST Yeast plasmid vectors contain the same basic features as E. 4). There are two primary types of replicators used in yeast plasmid vectors, autonomously replicating sequences (ARS) derived from the yeast chromosomes and the natural yeast 2µm plasmid replicator.

Download PDF sample

Rated 4.18 of 5 – based on 24 votes