By Liang-Fu Zhou, Guohua Xi, Xian-Cheng Chen, Richard F. Keep, Feng-Ping Huang, Ya Hua, Yi-Cheng Lu, Karin Muraszko
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Extra resources for Cerebral Hemorrhage (Acta Neurochirurgica Supplementum 105)
Chen1 , J. H. Zhang1;2;3 , J. Tang1;4 1 Department Department 3 Department 4 Department 5 Department 2 of of of of of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA, USA Neurosurgery, Loma Linda University, Loma Linda, CA, USA Anesthesiology, Loma Linda University, Loma Linda, CA, USA Physiology, Chongqing Medical University, Chongqing, China Anesthesiology, Krasnoyarsk State Medical University, Krasnoyarsk, Russia Summary The use of exogenous superoxide dismutase (SOD) and catalase (CAT) has been previously evaluated against various reactive oxygen speciesmediated brain injuries, especially those associated with ischemia= reperfusion.
B) MDC staining of control neurons and ferrous iron-treated neurons. Note the MDC-labeled vacuoles in the latter. Scale bar ¼ 100 mm oxidative stress can induce autophagic cell death . Therefore, iron may be an important trigger for ICHinduced autophagic cell death. LC3 has been used as a marker of autophagy. LC3 has 2 forms: type I is cytosolic and type II is membranebound. During autophagy, LC3 type II is increased by conversion from LC3 type I. The ratio of LC3-II to LC3-I is correlated with the extent of autophagosome formation .
The second antibody was goat anti-rabbit IgG (Bio-Rad Laboratories; 1:2500 dilution). The antigen-antibody complexes were visualized with a chemiluminescence system and exposed to Kodak X-OMAT ﬁlm. 61 (National Institutes of Health, Bethesda, MD). 05 mM MDC in PBS for 30 min at 37 C. , Melville, NY). Statistical analysis All data in this study are presented as mean Æ SD. Data were analyzed using Student t-test and analysis of variance (ANOVA). 05. Fig. 1. Ferrous iron induces conversion of LC3-I to LC3-II.