Baculovirus and Insect Cell Expression Protocols by David W. Murhammer (auth.), David W. Murhammer (eds.)

By David W. Murhammer (auth.), David W. Murhammer (eds.)

Baculovirus Expression Protocols is an in depth consultant for utilizing the baculovirus expression vector method (BEVS) and/or insect cells to supply recombinant proteins. approaches for the advance and creation of baculovirus pesticides at either laboratory and massive scale are defined within the form of step by step structure that the tools in Molecular Biology™ sequence helped standardize. This booklet is a one-stop resource for info on baculoviruses, and comprises sections on utilizing transformed baculoviruses to precise genes in mammalian cells, utilizing Drosophila telephone traces, separating new mobile strains, and constructing serum-free medium. particular functions, together with utilizing baculovirus and bug cellphone approach to check apoptosis, also are mentioned. This up to date and accelerated variation of Baculovirus Expression Protocols offers an ideal lab guide for biochemists, molecular biologists, biochemical engineers, and others for utilizing the BEVS or insect cellphone traces to supply recombinant proteins.

Show description

Read Online or Download Baculovirus and Insect Cell Expression Protocols PDF

Best biology books

Osteoporosis: Methods and Protocols

Lately, the examine of bone cells and tissues on the mobile and molecular degrees in a number of versions has revolutionized the sector. In Osteoporosis: equipment and Protocols, top scientists from all over the world proportion their step by step laboratory protocols for learning bone biology. the themes lined during this quantity comprise in vitro versions, in vivo types applied for drug trying out, tissue engineering and osteoporosis experiences in both gender, state of the art molecular recommendations to evaluate unmarried genes or for international genomic research, strong imaging concepts, and lots of extra.

Molecular Mechanisms in Bioenergetics

This publication summarises present wisdom of the constitution, function,biosynthesis and law of energy-transducing enzymes inmitochondria, chloroplasts and micro organism. all the twenty chapters is written by means of best specialists of their box, and Prof. Ernster has ensured that the publication as a complete supplies a well-integrated photograph of the current country of data of the sphere at its diversified degrees and complexities.

Additional info for Baculovirus and Insect Cell Expression Protocols

Sample text

Virol. 69, 156–165. 70 Pullen, S. S. and Friesen, P. D. (1995) The CAGT motif functions as an initiator 70. element during early transcription of the baculovirus transregulator ie-1. J. Virol. 69, 3575–3583. 71 Friesen, P. D. (1997) Regulation of baculovirus early gene expression, in The 71. Baculoviruses, (Miller, L. ),Plenum Press, New York, pp. 141–191. 72 Tomalski, M. , Wu, J. , and Miller, L. K. (1988) The location, sequence, 72. transcription, and regulation of a baculovirus DNA polymerase gene.

Virol. 66, 6903– 6911. , van Dinten, L. , et al. (1999) Specificity of polyhedrin in 33. the generation of baculovirus occlusion bodies. J. Gen. Virol. 80, 1045–1053. 34 Minion, F. , Coons, L. , and Broome, J. R. (1979) Characterization of the 34. polyhedral envelope of the nuclear polyhedrosis virus of Heliothis virescens. J. Invertebr. Pathol. 34, 303–307. 35 Whitt, M. A. and Manning, J. S. (1988) A phosphorylated 34-kDa protein and a 35. subpopulation of polyhedrin are thiol linked to the carbohydrate layer surrounding a baculovirus occlusion body.

15 15. 16 16. 17 17. 18 18. 19 19. 20 20. 21 21. 43 neutralization, comparative infectivity, and in vitro growth studies. J. Virol. 19, 820–832. Volkman, L. E. (1983) Occluded and budded Autographa californica nuclear polyhedrosis virus: Immunological relatedness of structural proteins. J. Virol. 46, 221–229. Volkman, L. E. and Goldsmith, P. A. (1984) Budded Autographa californica NPV 64K protein: Further biochemical analysis and effects of postimmunoprecipitation sample preparation conditions.

Download PDF sample

Rated 4.73 of 5 – based on 5 votes