By T F Kruger; Daniel R Franken
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Penetration of human spermatozoa into the human zona pellucida and the zonafree hamster egg: a study of fertile donors and infertile patients. In Keel BA, Webster BW, eds. Characterization of the biologic activities of a recombinant human zona pellucida protein 3 expressed in human ovarian teratocarcinoma (PA1) cells. Outcome of intracytoplasmic sperm injection in azoospermic patients: stressing the liaison between the urologist and reproductive medicine specialist. 11 showed the AI to be a more sensitive parameter, compared with normal sperm morphology evaluated according to strict criteria, in the prediction of expected in vitro fertilization rates of ≥50%; the use of the AI was found to be particularly useful in the P pattern group of patients.
The ‘feathering’ technique, whereby the edge of a second slide is used to drag a drop of semen along the surface of the cleaned slide to make thin smears of spermatozoa, was used12. Evaluation of the sperm smear quality was made under 1000× magnification (10× ocular, 100× objective) and included recordings of the number of sperm per microscopic field in the four corners and center of each slide. To ensure optimal slide quality, we use the following standard protocol during slide preparations: (1) Frosted, precleaned glass slides with ground edges are used at all times.
To ensure optimal slide quality, we use the following standard protocol during slide preparations: (1) Frosted, precleaned glass slides with ground edges are used at all times. 3). 4). Sperm morphology training Sperm morphology assessment is a subjective method, and intensive training is therefore needed to ensure that the technologists possess the required evaluation skills.