Anatomy Of The Dicotyledons Vol I by Metcalfe,C.R.; Chalk,L.

By Metcalfe,C.R.; Chalk,L.

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Biotechnol. 19, 45–50. , Breitkreutz, B. , Hon, G. , Myers, C. , Stark, C. et al. (2006). Comprehensive curation and analysis of global interaction networks in Saccharomyces cerevisiae. J. Biol. 5, 11. , Williams, B. , King, N. and Carroll, S. B. (2003). Genome-scale approaches to resolving incongruence in molecular phylogenies. Nature 425, 798–804. Ross, P. , Huang, Y. , Marchese, J. , Daniels, S. et al. (2004). Multiplexed protein quantitation in Saccharomyces cerevisiae using aminereactive isobaric tagging reagents.

And Tanaka, T. U. (2005). Molecular mechanisms of kinetochore capture by spindle microtubules. Nature 434, 987–994. , Pohlmann, R. and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793–1808.

Take 100 ml of this suspension, and spread onto a YPD agar plate using a sterile spreader. 3. 5 cm2 areas of the strains whose mating type is to be tested. These should have been freshly grown overnight prior to the patching. Use generous amounts (1–2 whole colonies) of the strains to be tested in order that their growth gets a head start over that of the RC629 lawn. 4. Results should be clear after 24–36 h. MATa strain patches will inhibit growth of RC629, and be surrounded by a cleared zone in the lawn (see Figure 1A).

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