By F. F. Nord
Advances in Enzymology and comparable parts of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative studies of the newest discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unequalled view of the ancient improvement of enzymology. The sequence deals researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic procedure, and their software in either the laboratory and undefined. every one quantity within the sequence beneficial properties contributions through prime pioneers and investigators within the box from world wide. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.
With its wide variety of issues and lengthy historic pedigree, Advances in Enzymology and similar parts of Molecular Biology can be utilized not just through scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by way of any scientist drawn to the invention of an enzyme, its houses, and its purposes.
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Biochem. ,38, 250 (1944). 34. , Proc. Exptl. Biol. , 55, 11 (1944). 35. Gladstone, G. , and Richardson, G. , Brit. J. Ezptl. , 16, 335 (1935). 36. Gordon, A. , Martin, A. J. , and Synge, R. L. ,37,313 (1943). 37. , Fortschr. , 1, 2 (1921); Chem. , 1921, I, 775. 38. Gunsalus, I. , and Bellamy, W. , J. , 155, 357 (1944). 39. Gunsalus, I. , Bellamy, W. , and Umbreit, W. , 155,685 (1944). 40. Gunsalus, I. , Niven, C. , and Sherman, J. , J. , 48,611 (1944). 40a. Gunsalus, I. , Umbreit, W. , Bellamy, W.
1 N sodium hydroxide a t 100”. 0 undergoing oxidation or hydrolysis a t room temperature. Treatment of codecarboxylase with nitrous acid, hypobromous acid, or benzoyl chloride has no effect on activity. E. F. GALE 24 5. Preparation of Codecarboxylase Concentrate A method of concentration of natural codecarboxylase has been worked out on the basis of these properties (33). Convenient starting material is dried yeast (brewers' or bakers') which is extracted a t 37" C. 75% baryta and the sediment then centrifuged down, leaving the bulk of the active material in the supernatant fluid.
VIII. Conclusions.. . . . . . . . . . . . . . . . . . . . . . . . . . . Bibliography. . . . ..... . 90 90 91 96 102 113 118 119 121 I. Introduction An understanding of the problems indicated by the title of the present study requires their treatment in relation to one another. By emphasizing the biochemical aspects involved, it is hoped that some light may be thrown on the chemical nature of various hitherto undefined biological processes.